Professional Bio

1963-1967 B.A. Biology   Oberlin College, Oberlin, OH  

1967-1969 M.Sc. Microbiology Yale University, New Haven, CT
Advisor  P.T. Magee. Threonine Deaminase of Saccharomyces cerevisiae.

1969-1972 Ph.D. Biochemistry University College London, London, England
Advisor Patricia H. Clarke, F.R.S., Phenylacetamidases in Pseudomonas aeruginosa.

1972-1976 Postdoctoral research in Molecular Biology, University of Colorado Health Sciences Center (UCHSC), Denver, CO.  With John R. Sadler (Department of Biophysics & Genetics). Escherichia coli lactose repressor-operator interaction.

1980-1981 Postdoctoral research in Virology, UCHSC, Denver, CO.  With Lewis I. Pizer (Department of Microbiology & Immunology). Regulation of Herpes simplex virus glycoprotein D (gD) gene by the ICP4 (Vmw175, IE3) protein.

Research & Scholarship

Major Scientific Interest:  Mechanisms of regulation of gene expression in prokaryotes and eukaryotes, particularly protein-DNA interactions controlling initiation of transcription.

Awards & Recognition

1967 Graduated Oberlin College Magna cum Laude and Phi Beta Kappa

1970-1972 National Science Foundation Predoctoral Fellow

1974-1976 National Institutes of Health Postdoctoral Fellow

2003 Regis College Faculty Lecturer of the year; March 12, 2004 presentation “Adventures in Genetics: Research with Model Systems”

Recent Extramural Funding

NIH AREA grant R15 AI 32665 "Gene regulation by Varicella zoster virus VP140 protein", Joan Betz, principal investigator (7-1-92 to 6-30-95), $75,000 direct costs; extended without additional funding to 6-30-96.

 

NSF DUE 9551978 "Fluorescent spectrometry in the undergraduate program at Regis University", Surendra Mahapatro, principal investigator; Joan Betz, co-principal investigator (7-1-95 to 6-30-97), $13,306 costs (matched by Regis).                        .

 

NIH AREA grant R15 DK/OD 52577 "Transcriptional competence of the PML-RAR fusion receptor", Joan Betz, principal investigator (6-1-97 to 5-31-00), $75,000 direct costs; extended without additional funding to 5-31-01.

 

NSF DUE 952711 "Integrated neuroscience laboratories", Gene Delay, principal investigator; Joan Betz, Surendra Mahapatro, and Clifford Barnes, co-principal investigators (6-1-00 to 5-31-02), $25,000 direct costs (matched by Regis). 

 

NIH RO1 grant GM38101 "Mechanisms of yeast transcription". Judith Jaehning, principal investigator (12-01-00 to 11-30-04) $800,000 total direct costs. Joan Betz, co-PI (summer salary).

 

NIH R15 GM076099 “Subunit interaction of components of the yeast Paf1 complex”. Joan Betz, principal investigator (1-1-06 to 12-31-08). $150,000 total direct costs (about $30,000 indirect);  extended without additional funding to 12-31-10.

Recent and current Intramural Funding

Regis SPARC (Sponsored Projects Academic Research Council) grant: "Genetic analysis of transcriptional regulation by the yeast Paf1/RNA polymerase II complex", Joan Betz, principal investigator (5-1-01 o 4-31-02) $3000 direct costs.

 

Regis SPARC grant: “Subunit interaction of components of the yeast Paf1 complex”. Joan Betz, principal investigator (5-1-05 to 4-30-06) $2642 direct costs.

 

Regis SPARC grants to undergraduate students: six different research students have each received up to the $500 (maximum) annual awards to support supplies for research and travel to scientific conferences (Cheryl Dunne, Bao T. Nguyen (2), Tom Deem (2), Brian Couch, Donovan Rigirozzi, Randal Davis). These students presented the results of their research at the annual Regis Student Research Symposia, and in three cases, presented at the West Coast Biological Sciences Undergraduate Research Symposia.

 

Regis Faculty Development Committee grants:

Travel grants:  since 1990, have received nearly annual awards of $800 to $1200 (maximum permitted) towards travel to a professional conference.

 

Small grants:  since 1990, have received nearly annual awards of $300 (maximum permitted) for purchase of special reagents, small equipment, prepared microscope slides, repair of equipment and other items to improve teaching and research.

 

Presentations:        

Betz, J.L. (June, 1994). Using in vitro transcription to analyze retinoic acid receptor activation of the RARb2 gene. European Molecular Biology Lab, Heidelberg, Germany. (seminar).

Betz, J.L. (April, 1995). Genetics and Ethics. Honors Program Symposium, Regis University, Denver, CO. (one of three speakers).

Betz, J.L. (May, 1995). DNA binding sites can contribute to gene activation by varicella zoster virus gene 62 protein and the homologous herpes simplex virus ICP4 protein. NIH AREA conference, Indianapolis, IN. (poster).

Betz, J.L. and Stunnenberg, H.G. (Oct., 1995). Positioning of the retinoic acid receptor on DR1 binding sites assessed by UV cross-linking. UCHSC Molecular Biology Program Retreat, Estes Park, CO. (poster).

 Betz, J.L., Sanguedolce, M.V., Leblanc, B.P. and Stunnenberg, H.G. (Aug., 1996). The promoter context is a decisive factor in establishing selective responsiveness to nuclear receptors. Second EMBL meeting on Transcription, Heidelberg, Germany. (poster).

Betz, J.L., Sanguedolce, M.V., Leblanc, B.P. and Stunnenberg, H.G. (Nov., 1996). The promoter context is a decisive factor in establishing selective responsiveness to nuclear receptors. UCHSC Molecular Biology retreat, Estes Park, CO. (poster).

Pizer, L.I., Buchler, P., Everett, R. and Betz, J.L. (Aug., 1997). Weight matrix analysis of specific DNA binding by ICP4. XXII International Herpesvirus Workshop, San Diego, CA. (poster).

Betz, J.L., Garcia-Jimenez, C. and Stunnenberg, H.G. (Nov., 1997). Characterization of the promyelocytic leukemia- retinoic acid receptor purified from vaccinia virus-infected cells.  UCHSC Molecular Biology Retreat, Estes Park, CO. (poster).

Betz, J.L., Chang, M., French-Cornay, D. and Jaehning, J. (Nov., 1998). Construction of specific knock-out mutations to analyze regulations by Paf1p in Saccharomyces cerevisiae UCHSC Molecular Biology Retreat, Estes Park, CO. (poster).

Betz, J.L. (March, 1999). Gene regulation by retinoic acid receptor and by its fusion protein PML-RAR that causes acute promyelocytic leukemia, Oberlin College, OH. (seminar).

Betz, J.L., Chang, M., Goyette, K. and Jaehning, J. (Aug., 1999). Genetic analysis of protein kinase C pathway signaling by the Paf1p/ RNA polymerase II complex. Mechanisms of Eukaryotic Transcription, Cold Spring Harbor Laboratory, NY (poster).

Betz, J.L., Chang, M., Goyette, K. and Jaehning, J. (Nov., 1999). Genetic analysis of protein kinase C pathway signaling by the Paf1p/ RNA polymerase II complex. UCHSC Molecular Biology Retreat, Estes Park, CO. (poster).

Betz, J.L., Chang, M., Goyette, K., Porter, S., Mueller, C. and Jaehning, J.A. (June, 2000). The yeast Paf1p/RNA polymerase II complex mediates protein kinase C signaling and is important for expression of cell cycle regulated genes. FASEB Summer Research Conference on Transcriptional regulation during cell growth, differentiation and development, Snowmass, CO. (poster).

Betz, J.L., Goyette, K., Chang, M. and Jaehning, J.A. (July, 2000). Genetic analysis of the role of the Paf1p/RNA polymerase II complex in the Pkc1p signaling pathway. Yeast Genetics and Molecular Biology Meeting, Seattle, WA. (poster).

Betz, J.L., Chang, M., Washburn, T., Porter, S. and Jaehning, J. (Nov., 2000). Genetic analysis of the role of the Paf1p/RNA polymerase II complex. UCHSC Molecular Biology Retreat, Estes Park, CO. (poster).

Betz, J.L. (June, 2001). Genetic analysis of the role of the Paf1p/RNA polymerase II complex. Dept. Biochemistry and Molecular Genetics, UCHSC. (seminar).

Betz, J.L., Chang, M., Washburn, T., Porter, S. and Jaehning, J. (Aug., 2001). Genetic analysis of the function of the Paf1p/RNA polymerase II complex. Conference on Mechanisms of Eukaryotic Transcription. Cold Spring Harbor Laboratory, NY. (poster).

Betz, J.L., Chang, M., Washburn, T., Porter, S. and Jaehning, J.A. (Nov., 2001). Expanded functional role of the yeast Paf1p/RNA polymerase II complex deduced from phenotypic analysis. UCHSC Molecular Biology retreat, Estes Park, CO. (poster).

Betz, J.L. Porter, S.E., Washburn, T.M. and Jaehning, J.A. (Aug., 2002) Suppression of pleiotropic phenotypes of paf1 by over-expresion of Swi4, Mbp1 and Rnr1 confirms the role of the Paf1/RNA polymerase II complex in cell cycle regulation. Yeast Genetics and Molecular Biology Meeting, Madison, WI. (poster).

Betz, J.L., Nguyen, B.T. and Jaehning, J.A. (Nov., 2002). High copy suppressors of Paf1 complex mutants. UCHSC Molecular Biology retreat, Winter Park, CO. (poster).

Betz, J.L., Nguyen, B.T. and Jaehning, J.A. (Aug., 2003). Suppressors of pleiotropic phenotypes of mutants of the Paf1/RNA polymerase II complex. Conference on Mechanisms of Eukaryotic Transcription. Cold Spring Harbor Laboratory, NY. (poster).

Betz, J.L., Nguyen, B.T. and Jaehning, J.A. (Nov., 2003). Suppressors of pleiotropic phenotypes of mutants of the Paf1/RNA polymerase II complex. The 14th Annual Molecular biology Retreat (UCHSC), Estes Park, CO. (poster).

Betz, J.L. (March, 2004). Adventures in Genetics: research with model systems. Regis University (invited seminar as Regis College Faculty Lecturer of the Year, 2003).

Betz, J.L., Nguyen, B.T. and Jaehning, J.A. (July, 2004). Over-expression of Cln2 or Cln3 suppresses loss of the Paf1/RNA polymerase II complex. Yeast Genetics and Molecular Biology Meeting, Seattle, WA. (poster).

Betz, J.L., Nguyen, B.T. and Jaehning, J.A. (Nov., 2004). Over-expression of Cln2 or Cln3 suppresses loss of the Paf1/RNA polymerase II complex. The 15th Annual Molecular biology Retreat (UCHSC), Winter Park, CO. (poster).

Betz, J.L., Hoffman, M.G., Nordick, K.M., Penheiter, K.L. and Jaehning, J.A. (Aug., 2005). The yeast Paf1 complex is required for association of cleavage and poly(A) factors with pol II. Mechanisms of Eukaryotic Transcription. Cold Spring Harbor Laboratory, NY. (poster).

Betz, J.L., Hoffman, M.G., Nordick, K.M., Campsteijn, C., Cohen, A., Jansen, P., Logie, C., Stunnenberg, H. and Jaehning, J.A. (July, 2006). Interactions of the Paf1 complex: Paf1C is required for association of cleavage and poly(A) factors with RNA polymerase II. Yeast Genetics and Molecular Biology Meeting, Princeton, NJ. (poster).

Betz, J.L., Hoffman, M.G., Nordick, K.M., Campsteijn, C., Cohen, A., Jansen, P., Logie, C., Stunnenberg, H. and Jaehning, J.A. (Nov., 2006). Interactions of the Paf1 complex: Paf1C is required for association of cleavage and poly(A) factors with RNA polymerase II. The 17th Annual Molecular biology Retreat (UCDHSC), Winter Park, CO. (poster).

Publications:

  1. Borle, A.B. and Loveday, J. (1968).  Effects of temperature, potassium and calcium on the electrical potential difference in HeLa cells.  Cancer Research 28, 2401-2405.
  2. Betz, J.L., Hereford, L.M. and Magee, P.T. (1971).  Threonine deaminases from Saccharomyces cerevisiae mutationally altered in regulatory properties.  Biochemistry 10, 1818-1824.
  3. Betz, J.L. and Clarke, P.H. (1972).  Selective evolution of phenylacetamide-utilizing strains of Pseudomonas aeruginosa.  J. Gen. Microbiol. 73, 161-174.
  4. Betz, J.L. and Clarke, P.H. (1972).  Growth of Pseudomonas species on phenylacetamide.  J. Gen. Microbiol. 75, 167-177.
  5. Betz, J.L., Brown, P.R., Smyth, M.J. and Clarke, P.H. (1974).  Evolution in action.  Nature (London) 247, 261-264.
  6. Walker, T.A., Betz, J.L., Olah, J. and Pace, N.R. (1975).  The nucleotide sequence of dolphin and bovine 5S ribosomal ribonucleic acid.  FEBS Lett. 54, 241-244.
  7. Betz, J.L. and Sadler, J.R. (1976).  Tight-binding repressors of the lactose operon.  J. Mol. Biol. 105, 293-319.
  8. Sadler, J.R., Tecklenburg, M., Betz, J.L., Goeddel, D.V., Yansura, D.G. and Caruthers, M.H. (1977).  Cloning of chemically synthesized lactose operators.  Gene I, 305-321.
  9. Sadler, J.R., Betz, J.L., Tecklenburg, M., Goeddel, D.V., Yansura, D.G. and Caruthers, M.H. (1978).  Cloning of chemically synthesized lactose operators, II. EcoRI-linkered operators.  Gene 3, 211-232.
  10. Sadler, J.R., Tecklenburg, M., Betz, J.L. (1980).  Plasmids containing many tandem copies of a synthetic lactose operator.  Gene 8, 279-300.
  11. O'Gorman, R.B., Ferguson, L., Betz, J.L., Sadler, J.R. and Matthews, K.S. (1981).  Determination of the ligand-binding characteristics of several tight-binding mutants of the lactose repressor protein.  Biochem. Biophys. Acta 653, 236-247.
  12. Betz, J.L. and Sadler, J.R. (1981).  Variants of a cloned synthetic operator.  I.  A palindromic dimer lactose operator derived from one strand of the cloned 40-based pair operator.  Gene 13, 1-12.
  13. Betz, J.L. and Sadler, J.R. (1981).  Variants of a cloned synthetic lactose operator.  II.  Chloramphenicol-resistant revertants retaining a lactose operator in the CAT gene of plasmid pBR325.  Gene 15, 187-200.
  14. Betz, J.L., Hill, T.M., Pizer, L.I., Peake, M.L. and Sadler, J.R. (1983).  Transcription from the BamHI-J fragment of Herpes simplex virus type 1 (KOS).  J.Virol. 47, 238-243.
  15. Ikura, K., Betz, J.L., Sadler, J.R. and Pizer, L.I. (1983).  RNAs transcribed from a 3.6-kilobase SmaI fragment of the short unique region of the Herpes simplex virus type 1 genome.  J. Virol. 48, 460-471.
  16. Sadler, J.R., Sasmor, H. and Betz, J.L. (1983).  A perfectly symmetric lac operator binds lac repressor very tightly.  Proc. Natl. Acad. Sci. USA 80, 6785-6789.
  17. Betz, J.L., Sadler, J.R. and Hare, D.L. (1984).  Regulated expression of the Herpes simplex type 1 thymidine kinase gene in Escherichia coli.  In Rapp, F. (ed), Herpesvirus, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 21, Alan R. Liss, Inc., New York, pp. 301-310.
  18. Hare, D.L., Sadler, J.R. and Betz, J.L. (1984).  Regulated high-level expression of the Herpes simplex virus type 1 thymidine kinase gene in Escherichia coliGene 32, 117-128.
  19. Hill, T.M., Sadler, J.R. and Betz, J.L. (1985).  Virion component of Herpes simplex virus type 1 KOS interferes with early shutoff of host protein synthesis induced by Herpes simplex virus type 2 186.  J. Virol. 56, 312-316.
  20. Betz, J.L. (1986).  Cloning and characterization of several negative-dominant and tight-binding mutants of lac repressor.  Gene 42, 283-292.
  21. Betz, J.L., Sasmor, H., Buck, F., Insley, M.Y. and Caruthers, M.H. (1986).  Base substitution mutations in the lac operator: in vivo and in vitro affinities for lac repressor.  Gene 50, 123-132.
  22. Pizer, L.I., Tedder, D.G., Betz, J.L., Wilcox, K.W. and Beard, P. (1986).  Regulation of transcription in vitro from Herpes simplex virus genes.  J. Virol. 60, 950-959.
  23. Betz, J.L. (1987).  Affinities of tight-binding lactose repressors for wild-type and pseudo‑operators.  J. Mol. Biol. 195, 495-504.
  24. Pizer, L.I., Mitchell, D.H., Bentele, B. and Betz, J.L. (1987).  A mammalian cell line designed to test the mutagenic activity of anti-Herpes nucleosides.  Int. J. Cancer 40, 114-121.
  25. Betz, J.L. and Fall, M.A. (1988).  Effects of dominant-negative lac repressor mutations on operator specificity and protein stability.  Gene 67, 147-158.
  26. Betz, J.L. and Sasmor, H.M. (1990).  Symmetric derivatives of lac operator:  effects of spacing and flanking sequence on affinity for lac repressor.  Gene 80, 1-6.
  27. Sasmor, H.M. and Betz, J.L. (1990).  lac repressor specific binding affects adjacent binding sites in covalently closed operator molecules.  Biochemistry 29, 9023-9028.
  28. Betz, J.L. and Wydoski, S. (1993).  Functional interaction of Varicella-zoster virus 140K protein with DNA sequence bound Herpes simplex virus ICP4 protein.  Virology 195, 793-797.
  29. Sanguedolce, V., Leblanc, B.P., Betz, J.L. and Stunnenberg, H.G. (1997).  The promoter context is a decisive factor in establishing selective responsiveness to nuclear receptors.  EMBO J. 16, 2861-2873.
  30. Stunnenberg, H.G., Garcia-Jimenez, C. and Betz, J.L. (1998). Leukemia: the sophisticated subversion of hematopoiesis by nuclear receptor oncoproteins. BBA (Reviews on Cancer) 1423 (1): F15-F33.
  31. Betz, J.L., Chang, M., Washburn, T.M., Porter, S.E., Mueller, C.L. and Jaehning, J.A. (2002). Phenotypic analysis of Paf1/ RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism. Mol. Gen. Genomics. 268: 272-285.
  32. Nordick, K., Hoffman, M.G., Betz, J.L. and Jaehning, J.A. (2008). Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II. Eukaryotic Cell 7: 1158-1167 (epub 2008 May 9).

Professional Memberships:

American Society for Microbiology   

American Association for the Advancement of Science

Genetics Society of America

National Association of Advisors for the Health Professions

Council for Undergraduate Research

Alpha Epsilon Delta (National Health Preprofessional Honor Society)

Beta Beta Beta (Biological honor society)